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The morpho-functional state of Vicious-Circle target organs in mice with the induced of citokindependent hepatitis

           M. Rogava*, L. Chigladze*, T. Sanikidze**, M. Chkhaidze***,
E. Guchua*, T. Bochorishvili*

*- National Center of Therapy
**- The Scientific and Research Institute of Experimental and Clinical Medicin of the Tbilisi State Medical University
***- The Chair of the Pathologic Anatomy of the Tbilisi State Medical University 

The model induced by Con-A is received for the investigation of basic processes of liver patho­logy, initiated by activation of lymphocytes, infiltration and characterized by DNA fragmentation, significant release of transamins and apoptosis. A number of studies showed that the release of cytokines by activated cells leads to the increase of NO through inactivation of NO-syntetasys, this can result in a negative hipotropism and myocardial injury. Therefore the simplicity of the mentioned experimental model fully satisfies our theoretical and practical needs on this stage of investigation.

The experiment was hold in 30 mice of pure line, weighing 150-200g, which had been in quarantine. The first group (20 mice) - 2 ml of Con-A were injected. The second – control group (10 mice) interperitoneally received physiologic solution (the doses calculated per 1kg. of weight). The experiment was hold in surgical department of the laboratory, observing all rules of aseptic and antiseptics were strictly observed. After 24 hours post Con-A injection the animals were decapitated and samples were taken from their heart, brain, liver and blood. Free-radical processes and NO were studied using spectrometric resoner with quartz diurer. The computer program and self -registration device were receiving signals of paramagnetic spectrum that were registered by spectrometer PE 1307.   The morphological changes on liver, brain and heart were studied by electronic and light (luminous) microscope. For measurement of contractile ability of contractile protein system we used glycerized heap (sample) of fibers obtained from myocardium of right and left ventricles

The study of paramagnetic spectrum revealed the strongly developed dysfunction in all the studied organs, the release of Fe contributes to inactivation of prolipherative processes, the high concentration of Mn+2 ions indicates to the inhibitive activity of adenociclasies which in its turn initiates inactivation of peroxides and also is the reason of adrenoreceptorial inactivation of the   blood elements. The appearance of paramagnetic signals of Mo+5 containing xanoxsidaze indicates the existence of the obstacle for transportation of elements in mitochondrial energetic function The function of a whole body as well as the function of the separate organs in the certain period – (during borderline state), are stable. Whenever in target organs conditions supporting pathological state develop. The general reactivity of body decreases, occurs damage in the balance between oxidative and antioxidative systems and the influence of the initiating factors contribute to development of vicious-circle which determines the gravity of pathological disorders in target organs, and their variety (destruction in brain, heart and liver are strongly developed in parencim as well as in microcirculation system. There are sighs of vacuole dystrophy, granular dystrophy and leikocytarial infiltration of the liver, sighs of atrophic changes of cardiomiocits in heart that are characterized by destruction of miophibriles and mitochondria and finally in the gliocits of brain anoxia- ischemic occur).                                                                                                                                                                                                                                

On the basis of the mentioned changes in comparison with the control group, the well expressed

decrease of contractile function of the left and right ventricle (38.8% and 46.8% respectively, p< 0.005) can be considered as a serious disorder.

The results of an experimental study confirmed the existence of pathomorphological changes re­gis­ter­ed in our clinical and authopcial studies of the materials obtained from patients with hepato viruses.

We assume that considering predictable morpho-functional complications of the main disease as well as the separate organs - borderline state - especially the viscous circle target organs in development of viscous circle will enable us to start preventive treatment on the earlier stage of dieses.

 

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