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Direct effect of Se and Rb on the capacity of restriction of the system
of contractile proteins during Dysfunction of myocardium developed
at the modeling cytokin dependant induced hepatitis
M.Rogava*, E. Guchua*, R. Gakhokidze**, T. Bochorishvili*
National Center of Therapy*,
Department of Bioorganic Chemistry of Tbilisi State University**

Heart injuries induced by the so-called cardio tropic and hepatotropic viruses are incessantly growing proceeding from the epidemic peculiarities of virus diseases, which are followed by decrease of contraction ability of a system of contractile proteins and formation of heart insufficiency syndrome in neonates, young and aged patients. We consider that the diseases proceeding with heart insufficiency syndrome in the 20-50-ies are one of the major causes of morbidity, invalidity and mortality. Drastic quantitative and percentage increase of chronic insufficiency syndrome in the population over middle age may be explained by the decrease of physiologic vitality of a body and by a tendency of the changes of the microelement specter alongside with the increase of biological age.
Experiments were performed on the pure-strain 150-200 g weight 30 albino rats which passed through quarantine. Decapitation of rats, taking the experimental material 24 hrs after injection of natural mitogene Con-A (20 albino rats the I group and 10 rats II-control group) were made in the operation block of the vivarium, by the observance of aseptic and antiseptic norms. Contraction of the system of contractile proteins of myocardium was defined on tensometer in auctonic regime (at the terms of variable charge). We performed measuring of few parallel threads according to the method of N.V.Korsanov et al 1973 and average data of its tension was calculated for the cross-section of natural thread, according to the formulae of R.E.Rarnavy 1954. Tension developed by glycerinized fiber bundles of ventricles, observed after 24 hrs of experiment was sharply decreased compared with the control group. By 1,72 and 1,49 nm/mm2 compared with the left ventricle, and by 2,81-2,8 mn/mm2 compared with the norm, that is by 38,8% and in the right ventricle – by 46,8% (p<0,005).
Tension developed by glycerinized fiber bundle of myocardium after re-incubation of preparation No 1 - Rameri and No 2 – Ramsel (see Fig. 1) sharply increased compared with the control group, correspondingly by 54 and 55% and in both cases exceeded the norm, respectively by 15 and 16%. Growth of contraction capacity of the system of contractile proteins was less sharply expressed in the cases of the preparation No 3 (Neoselen) and No 4 (Rb) and correspondingly formed 31 and 20% and compared with the control group did not reach normal indices.
Experimental material has clearly proved the positive effect of the above stated microelements on the capacity of restoration of decreased contraction function of myocardium.
According to our opinion, our own clinical experience and the experimental data we may conclude that in the patients suffering from heart insufficiency it is expedient to define microelements and B group (including B2) vitamins and to make their further correction to prevent heart insufficiency and to improve state of patients and the prognosis.

 

Literature:
1.     Mosulishvili M., Shonia N., Ginturi E.  INAA Results for Metal Traces in Human Whole Blood, Frequency and Age Characteristics of the Distribution.  Journal of Radioanalytical and Nuclear Chemistry Articles, 88/1(1985)121-134.
2.     Kipshidze N., Rogava M., Treatment of Heart Failure in Dilated Cardiomyopathy, 1996.

3.      მ. როგავა 2001 “კარდიომიოპათიის მქონე ავადმყოფეში მანკიერი წრის კლინიკური ასპექტები” შრომათა კრებული II ტომი, “შინაგანი მედიცინის მიღწევები და პრობლემები-2001” გვ. 6-9.
4.      როგავა მ., თავხელიძე თ., ჭოლაძე ა., ბოჭორიშვილი თ., ქობლიანიძე ლ.  სოლკოსერილის ეფექტურობა კარდიო- და ჰეპატოტროპული ვირუსული ინფექციებით შეპყრობილ ავადმყოფებში ბაზისური თერაპიისა და ჰელიუმ-ნეონის ლაზერის ინტრავენური ზემოქმედების ფონზე.  „კარდიოლოგია და შინაგანი მედიცინა - XXI”, #1, 23-26.


6.     მ. როგვა, ლ. ჭიღლაძე, თ. სანიკიძე, მ.ჩხაიძე, ე.გუჩუა, თ. ბოჭორიშვილი. “მანკიერი წრის სამიზნე ორგანოთა მორფო-ფუნქციური მდგომარება ვირთაგვებში ციტოკინდამოკიდებული ინდუცირებული ჰეპატიტის მოდელირებისას” ჟურნ. “კარდიოლოგია და შინაგანი მედიცინა”. 3, 2003. გვ. 153-165

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